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Merry Christmas everyone!!

thevampirediaries:

I can’t stay long, but I just wanted to say a quick thanks for the all the messages, and for anyone who put me in their follow forever/favourite blog posts! It’s already Christmas in Australia, but I think it’s still Christmas Eve in some parts of the world… either way, hope you’re all having a great time and spending it with people you love :)

http://informahealthcare.com/doi/abs/10.1080/13880200601026317

Abstract

A methanol extract of the Psophocarpus tetragonolobus. (L.) DC (Leguminosae) leaves was screened for antimicrobial activity against Pseudomonas aeruginosa.. Antimicrobial tests were carried out using the disk diffusion assay and broth dilution method against Pseudomonas aeruginosa.. The extract showed favorable antimicrobial activity against Pseudomonas aeruginosa. with a minimum inhibitory concentration (MIC) value of 2.55 mg/mL. Apart from the antibacterial effects, imaging using scanning electron microscopy (SEM) was done to determine the major alterations in the microstructure of the Pseudomonas aeruginosa.. The main abnormalities noted via SEM studies were alterations in morphology and complete collapse of the bacterial cells after 36 h of exposure to the extract. The effect of the extract on the growth profile of the bacteria was also examined. The extract changed the normal growth profile of Pseudomonas aeruginosa., thus confirming the bactericidal effect of the extract on Pseudomonas aeruginosa.. Furthermore, the extract was also tested against brine shrimp for toxicity. The extract exhibited no significant toxicity (LC50 = 1.30 mg/mL) against Artemia salina. L. (Artemiidae).

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Abstract

Legumes are considered to be a very good source of polyphenolic compounds that may act as chemopreventive agents, especially by their antioxidant properties. However, many of the chemopreventive properties may depend on the concentrations of the phytochemical compounds because potent antioxidant polyphenolic compounds may have pro-oxidant properties and negatively affect cell growth and viability. Thus, the aim of the present study was to assess the possible effect of two potent antioxidant Greek Leguminosae family plant extracts on the growth of a specific cancer cell line and its antioxidant defense cell system. Aqueous extracts of aerial parts of Lathyrus laxiflorus and Phaseolus vulgaris plants were initially examined for their cytotoxicity on the Hep2 cancer cell line at concentrations that possess potent antioxidant properties (100, 400, and 800 microg/mL). After a 24-hour incubation with the extracts, only L. laxiflorus plant extract exhibited the ability to inhibit the cell growth at 400 and 800 microg/mL by 57% and 74%, respectively, whereas P. vulgaris extract had no effect on cell growth at any of the tested concentrations. Noncytotoxic concentrations, 100 microg/mL L. laxiflorus and 800 microg/mL P. vulgaris extract, were used for 2-, 12-, and 24-hour incubation of the cells. The influence of the extracts on the antioxidant defense system of the cells was assessed by measuring the total antioxidant capacity (TAC) of the cells, the catalase (CAT) activity, and the concentrations of reduced glutathione, the oxidized form of glutathione, and thiobarbituric-reactive substances (TBARS) in all times of incubation with the cells. From the results obtained, it seems that only L. laxiflorus extract induces oxidative stress in the cells by reducing TAC and CAT activity and by inducing TBARS, especially with 2 and 12 hours of incubation. P. vulgaris extract reduced only TAC at 2 hours of incubation, indicating also a mild induction of oxidative stress. These results imply that potent antioxidant extracts, beyond a critical concentration, may induce oxidative stress even with no apparent cytotoxicity in cells.

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vanniecruz:

kbombdotcom:

therock1300:

jrmeriales:

(via omgellyssababy, trishamarieee)
Reblog if you’re attracted: Daniel Radcliffe, Rupert Grint ,Tom Felton, Matthew Lewis, James Phelps, or Oliver Phelps
When someone…

Calls me ugly:

Calls me sexy:

Calls me cute:

Calls me fat:

Tells me I’m wrong:

Tells me a boring story:

Tells me they love me:

Tells my parents about what I did over the weekend:

And when you reblog this I’ll be like: